Fluorescent analysis for bioindication of ozone on unicellular models.
Identifieur interne : 001D61 ( Main/Exploration ); précédent : 001D60; suivant : 001D62Fluorescent analysis for bioindication of ozone on unicellular models.
Auteurs : Victoria V. Roshchina [Russie] ; V A Yashin ; A V KuchinSource :
- Journal of fluorescence [ 1573-4994 ] ; 2015.
Descripteurs français
- KwdFr :
- Agents neuromédiateurs (analyse), Amines (analyse), Equisetum (croissance et développement), Equisetum (métabolisme), Fluorescence (MeSH), Humains (MeSH), Modèles théoriques (MeSH), Ozone (analyse), Plantes (composition chimique), Plantes (métabolisme), Pollen (croissance et développement), Pollen (métabolisme), Spectrométrie de fluorescence (MeSH), Spores (croissance et développement), Spores (métabolisme).
- MESH :
- analyse : Agents neuromédiateurs, Amines, Ozone.
- composition chimique : Plantes.
- croissance et développement : Equisetum, Pollen, Spores.
- métabolisme : Equisetum, Plantes, Pollen, Spores.
- Fluorescence, Humains, Modèles théoriques, Spectrométrie de fluorescence.
English descriptors
- KwdEn :
- Amines (analysis), Equisetum (growth & development), Equisetum (metabolism), Fluorescence (MeSH), Humans (MeSH), Models, Theoretical (MeSH), Neurotransmitter Agents (analysis), Ozone (analysis), Plants (chemistry), Plants (metabolism), Pollen (growth & development), Pollen (metabolism), Spectrometry, Fluorescence (MeSH), Spores (growth & development), Spores (metabolism).
- MESH :
- chemical , analysis : Amines, Neurotransmitter Agents, Ozone.
- chemistry : Plants.
- growth & development : Equisetum, Pollen, Spores.
- metabolism : Equisetum, Plants, Pollen, Spores.
- Fluorescence, Humans, Models, Theoretical, Spectrometry, Fluorescence.
Abstract
Unicellular model plant systems (vegetative microspores of horsetail Equisetum arvense and pollen of six plant species Corylus avellana, Dolichothele albescens Populus balsamifera, Salix caprea, Saintpaulia ionantha, Tulipa hybridum, on which autofluorescence and fluorescence after histochemical treatment studied, have been represented as bioindicators of ozone. It has found that low doses of ozone 0.005 or 0.008 μl/l did not affect or stimulate the autofluorescence of the samples with the ability to germinate in an artificial medium. In higher ozone concentrations (0.032 μl/l) either the decrease in the intensity of the emission or changing in the position of the maxima in the fluorescence spectrum (new 515-520 nm maximum characteristic for the green-and yellow area has appeared) were observed. In dose of 0.2 μl/l, higher than above the threshold of danger to human health, autofluorescence in all samples fell down to up to zero, and there was no the ability to germinate. In this case the formation of lipofuscin-like compounds fluoresced in blue with maxima from 440 to 485 nm was observed. Stress metabolites, known as neurotransmitters biogenic amines, were found in treated cells as determined on the characteristic fluorescence at 460-480 nm in the samples after a specific histochemical reactions for catecholamines (with glyoxylic acid) or for histamine (with o-phthalic aldehyde). Increased intensity of the emission under the treatment with ozone (total doses from 0.012 to 0.032 μl/l) was associated with an increase in the concentrations of catecholamines and histamine. The fluorescent analysis on undamaged cells-possible bioindicators of ozone can be useful in ecomonitoring for earlier warning about health hazardous concentrations of this compound in the air.
DOI: 10.1007/s10895-015-1540-2
PubMed: 25779939
Affiliations:
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Roshchina</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Cell Biophysics RAS, Institutskaya str. 3, Pushchino, Moscow Region, 142290, Russia, roshchinavic@mail.ru.</nlm:affiliation><country wicri:rule="url">Russie</country><wicri:regionArea>Institute of Cell Biophysics RAS, Institutskaya str. 3, Pushchino, Moscow Region, 142290, Russia</wicri:regionArea><wicri:noRegion>Russia</wicri:noRegion></affiliation></author><author><name sortKey="Yashin, V A" sort="Yashin, V A" uniqKey="Yashin V" first="V A" last="Yashin">V A Yashin</name></author><author><name sortKey="Kuchin, A V" sort="Kuchin, A V" uniqKey="Kuchin A" first="A V" last="Kuchin">A V Kuchin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25779939</idno><idno type="pmid">25779939</idno><idno type="doi">10.1007/s10895-015-1540-2</idno><idno type="wicri:Area/Main/Corpus">001D78</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001D78</idno><idno type="wicri:Area/Main/Curation">001D78</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001D78</idno><idno type="wicri:Area/Main/Exploration">001D78</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fluorescent analysis for bioindication of ozone on unicellular models.</title><author><name sortKey="Roshchina, Victoria V" sort="Roshchina, Victoria V" uniqKey="Roshchina V" first="Victoria V" last="Roshchina">Victoria V. Roshchina</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Cell Biophysics RAS, Institutskaya str. 3, Pushchino, Moscow Region, 142290, Russia, roshchinavic@mail.ru.</nlm:affiliation><country wicri:rule="url">Russie</country><wicri:regionArea>Institute of Cell Biophysics RAS, Institutskaya str. 3, Pushchino, Moscow Region, 142290, Russia</wicri:regionArea><wicri:noRegion>Russia</wicri:noRegion></affiliation></author><author><name sortKey="Yashin, V A" sort="Yashin, V A" uniqKey="Yashin V" first="V A" last="Yashin">V A Yashin</name></author><author><name sortKey="Kuchin, A V" sort="Kuchin, A V" uniqKey="Kuchin A" first="A V" last="Kuchin">A V Kuchin</name></author></analytic><series><title level="j">Journal of fluorescence</title><idno type="eISSN">1573-4994</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amines (analysis)</term><term>Equisetum (growth & development)</term><term>Equisetum (metabolism)</term><term>Fluorescence (MeSH)</term><term>Humans (MeSH)</term><term>Models, Theoretical (MeSH)</term><term>Neurotransmitter Agents (analysis)</term><term>Ozone (analysis)</term><term>Plants (chemistry)</term><term>Plants (metabolism)</term><term>Pollen (growth & development)</term><term>Pollen (metabolism)</term><term>Spectrometry, Fluorescence (MeSH)</term><term>Spores (growth & development)</term><term>Spores (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agents neuromédiateurs (analyse)</term><term>Amines (analyse)</term><term>Equisetum (croissance et développement)</term><term>Equisetum (métabolisme)</term><term>Fluorescence (MeSH)</term><term>Humains (MeSH)</term><term>Modèles théoriques (MeSH)</term><term>Ozone (analyse)</term><term>Plantes (composition chimique)</term><term>Plantes (métabolisme)</term><term>Pollen (croissance et développement)</term><term>Pollen (métabolisme)</term><term>Spectrométrie de fluorescence (MeSH)</term><term>Spores (croissance et développement)</term><term>Spores (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Amines</term><term>Neurotransmitter Agents</term><term>Ozone</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Agents neuromédiateurs</term><term>Amines</term><term>Ozone</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Plantes</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Equisetum</term><term>Pollen</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Equisetum</term><term>Pollen</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Equisetum</term><term>Plants</term><term>Pollen</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Equisetum</term><term>Plantes</term><term>Pollen</term><term>Spores</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fluorescence</term><term>Humans</term><term>Models, Theoretical</term><term>Spectrometry, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fluorescence</term><term>Humains</term><term>Modèles théoriques</term><term>Spectrométrie de fluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Unicellular model plant systems (vegetative microspores of horsetail Equisetum arvense and pollen of six plant species Corylus avellana, Dolichothele albescens Populus balsamifera, Salix caprea, Saintpaulia ionantha, Tulipa hybridum, on which autofluorescence and fluorescence after histochemical treatment studied, have been represented as bioindicators of ozone. It has found that low doses of ozone 0.005 or 0.008 μl/l did not affect or stimulate the autofluorescence of the samples with the ability to germinate in an artificial medium. In higher ozone concentrations (0.032 μl/l) either the decrease in the intensity of the emission or changing in the position of the maxima in the fluorescence spectrum (new 515-520 nm maximum characteristic for the green-and yellow area has appeared) were observed. In dose of 0.2 μl/l, higher than above the threshold of danger to human health, autofluorescence in all samples fell down to up to zero, and there was no the ability to germinate. In this case the formation of lipofuscin-like compounds fluoresced in blue with maxima from 440 to 485 nm was observed. Stress metabolites, known as neurotransmitters biogenic amines, were found in treated cells as determined on the characteristic fluorescence at 460-480 nm in the samples after a specific histochemical reactions for catecholamines (with glyoxylic acid) or for histamine (with o-phthalic aldehyde). Increased intensity of the emission under the treatment with ozone (total doses from 0.012 to 0.032 μl/l) was associated with an increase in the concentrations of catecholamines and histamine. The fluorescent analysis on undamaged cells-possible bioindicators of ozone can be useful in ecomonitoring for earlier warning about health hazardous concentrations of this compound in the air. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25779939</PMID><DateCompleted><Year>2016</Year><Month>03</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-4994</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>3</Issue><PubDate><Year>2015</Year><Month>May</Month></PubDate></JournalIssue><Title>Journal of fluorescence</Title><ISOAbbreviation>J Fluoresc</ISOAbbreviation></Journal><ArticleTitle>Fluorescent analysis for bioindication of ozone on unicellular models.</ArticleTitle><Pagination><MedlinePgn>595-601</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10895-015-1540-2</ELocationID><Abstract><AbstractText>Unicellular model plant systems (vegetative microspores of horsetail Equisetum arvense and pollen of six plant species Corylus avellana, Dolichothele albescens Populus balsamifera, Salix caprea, Saintpaulia ionantha, Tulipa hybridum, on which autofluorescence and fluorescence after histochemical treatment studied, have been represented as bioindicators of ozone. It has found that low doses of ozone 0.005 or 0.008 μl/l did not affect or stimulate the autofluorescence of the samples with the ability to germinate in an artificial medium. In higher ozone concentrations (0.032 μl/l) either the decrease in the intensity of the emission or changing in the position of the maxima in the fluorescence spectrum (new 515-520 nm maximum characteristic for the green-and yellow area has appeared) were observed. In dose of 0.2 μl/l, higher than above the threshold of danger to human health, autofluorescence in all samples fell down to up to zero, and there was no the ability to germinate. In this case the formation of lipofuscin-like compounds fluoresced in blue with maxima from 440 to 485 nm was observed. Stress metabolites, known as neurotransmitters biogenic amines, were found in treated cells as determined on the characteristic fluorescence at 460-480 nm in the samples after a specific histochemical reactions for catecholamines (with glyoxylic acid) or for histamine (with o-phthalic aldehyde). Increased intensity of the emission under the treatment with ozone (total doses from 0.012 to 0.032 μl/l) was associated with an increase in the concentrations of catecholamines and histamine. The fluorescent analysis on undamaged cells-possible bioindicators of ozone can be useful in ecomonitoring for earlier warning about health hazardous concentrations of this compound in the air. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Roshchina</LastName><ForeName>Victoria V</ForeName><Initials>VV</Initials><AffiliationInfo><Affiliation>Institute of Cell Biophysics RAS, Institutskaya str. 3, Pushchino, Moscow Region, 142290, Russia, roshchinavic@mail.ru.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yashin</LastName><ForeName>V A</ForeName><Initials>VA</Initials></Author><Author ValidYN="Y"><LastName>Kuchin</LastName><ForeName>A V</ForeName><Initials>AV</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>03</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Fluoresc</MedlineTA><NlmUniqueID>9201341</NlmUniqueID><ISSNLinking>1053-0509</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000588">Amines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018377">Neurotransmitter Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>66H7ZZK23N</RegistryNumber><NameOfSubstance UI="D010126">Ozone</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000588" MajorTopicYN="N">Amines</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020829" MajorTopicYN="N">Equisetum</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005453" MajorTopicYN="N">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008962" MajorTopicYN="Y">Models, Theoretical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018377" MajorTopicYN="N">Neurotransmitter Agents</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010126" MajorTopicYN="N">Ozone</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011058" MajorTopicYN="N">Pollen</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013050" MajorTopicYN="N">Spectrometry, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013170" MajorTopicYN="N">Spores</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>11</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>02</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25779939</ArticleId><ArticleId IdType="doi">10.1007/s10895-015-1540-2</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Environ Pollut. 2004 Oct;131(3):347-54</Citation><ArticleIdList><ArticleId IdType="pubmed">15261397</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Health Perspect. 1978 Dec;27:139-47</Citation><ArticleIdList><ArticleId IdType="pubmed">738233</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Pollut. 2011 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2006;139(4):294-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16491015</ArticleId></ArticleIdList></Reference><Reference><Citation>J Fluoresc. 2004 Nov;14(6):745-50</Citation><ArticleIdList><ArticleId IdType="pubmed">15649027</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Rev Allergy. 1985 Jul;3(3):291-318</Citation><ArticleIdList><ArticleId IdType="pubmed">3893674</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Russie</li></country></list><tree><noCountry><name sortKey="Kuchin, A V" sort="Kuchin, A V" uniqKey="Kuchin A" first="A V" last="Kuchin">A V Kuchin</name><name sortKey="Yashin, V A" sort="Yashin, V A" uniqKey="Yashin V" first="V A" last="Yashin">V A Yashin</name></noCountry><country name="Russie"><noRegion><name sortKey="Roshchina, Victoria V" sort="Roshchina, Victoria V" uniqKey="Roshchina V" first="Victoria V" last="Roshchina">Victoria V. 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